【Experimental Log】2015/2/22-23

10 Sample:a1 (B. aristatoserrulata), a2 (B. aristatoserrulata), a5 (B. aristatoserrulata, not sure), r1 (B. ravenii), r2 (B. ravenii), r3 (B. ravenii), r4 (B. ravenii), r⑤ (B. ravenii), a① (B. aristatoserrulata, 大鬼湖畔), a② (B. aristatoserrulata, 大鬼湖畔). p.s : 1. the number in circle (ex: ②), that is I marked. 2. These samples are from 大小鬼湖 by Brendan. 3. I should use my serial number.

DNA extraction: EasyLid (2/22, with Brendan). 

Polymease Chain Reaction (PCR): (2/23, with Brendan) 試做自己的一個sample:a5 (Is there DNA inside ?) 其餘的做學長的Sample。結果不佳。a5沒有P出來。

Next: DNA測濃度。(分光光度計) 確定是否有DNA和是否濃度足夠。若加入上次和宜軒一起的兩個sample: CP68 (B. kawakamii) 和 CP (B. aristatoserrulata),總共有12個sample,需從中找出兩個可以上機的樣本,3/2需要使用。

初四一從高雄外婆家回來,便直接回實驗室了。這天 (2/22) 是Brendan的29歲生日。尼泊爾王子的小檗paper出爐了 (Systematics and biogeography of Berberis s.l. inferred from nuclear ITS and chloroplast ndhF gene sequences),Brendan嫌棄得很。Strictly speaking, it’s my first time to do MY experiment, I need more practice.  3/2 (Mon) PM 2:00, 農藝系做實驗

濃度問題:DNA濃度是否足夠?

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