【Experimental Log】2015/7/8-9

8 Sample889b② (B. breviesepala), 875a③ (B. aristatoserrulata), CP93 (B. ravenii), CP99 (B. hayatana), CP104 (B. breviesepala), CP113 (B. schaaliae), CP153 (B. mingetsensis), CP149 (B. mingetsensis) [CP149太髒,最後回溶再過colum時,溶不掉,又有步驟上的錯誤。]

DNA extraction:CTab (未秤重,目視約兩片正常大小葉子,揉碎直接用鋼珠打碎。) 在65度時放入搖晃機中,約莫一小時,此次只用震盪機 (50 1/s*3 mins)*3 (一顆大、兩顆中鋼珠),開始前要將葉子先撥碎。總體積用50 (-1) ug去溶。皆有過Colum。

NanoDrop (曲老師實驗室)889b② (B. breviesepala):308.8 ug/mL, 1.89 260/280, 2.28 260/230, 875a③ (B. aristatoserrulata):169.6 ug/mL, 1.95 260/280, 2.7 260/230, CP93 (B. ravenii):84.6 ug/mL, 1.85 260/280, 2.02 260/230, CP99 (B. hayatana):81.8 ug/mL, 1.85 260/280, 2.54 260/230, CP104 (B. breviesepala):69.5 ug/mL, 1.82 260/280, 2.09 260/230, CP113 (B. schaaliae):- ug/mL, – 260/280, – 260/230, CP153 (B. mingetsensis):95.4 ug/mL, 1.87 260/280, 2.4 260/230, CP149 (B. mingetsensis):- ug/mL, – 260/280, – 260/230。[波後都有點小崎嶇。]

Note:C用完了,要配藥了。我的心頭好花蓮小檗,這次怎麼沒有出來呢?有夠想你欸!Brenden學長倫太文山那批,不知為何保存的很好 (已詢問保存方法和狀態)。繼續加油。暑假預計8/17-8/25去日本九州,也找學長爬山。

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