【Experimental Log】2015/9/16-17

4 Sample:A1 (B. apicola), A2 (B. apicola), A3 (B. apicola), M1 (B. mori)。

DNA extraction:CTab (未秤重,目視約兩片正常大小葉子,揉碎直接用鋼珠打碎。) 在65度時放入搖晃機中,約莫一小時,此次只用震盪機 (50 1/s*3 mins)*3 (一顆大、兩顆中鋼珠),開始前要將葉子先撥碎。總體積用50 (-1) ug去溶:A1和A3(-2)。皆有過Colum。

NanoDrop (曲老師實驗室)A1 (B. apicola)473.7 (468.2) ug/mL, 1.75 (1.75) 260/280, 1.23 (1.23) 260/230, A2 (B. apicola)51.7 ug/mL, 1.86 260/280, 2.40 260/230, A3 (B. apicola)69.3 (-) ug/mL, 1.85 (-) 260/280, 2.46 (-) 260/230, M1 (B. mori)56.2 ug/mL, 1.89 260/280, 2.56 260/230。

Note:M1 (B. mori) 是英國老爺爺的新種,sample來自英國。A1、A2和A3,都是從玉山排雲山莊旁採下來的(感謝嘉大學妹協助),其皆原為高地小檗,在2014年,油脂把它和高山小檗合併,皆稱為B. breviesepala