【Experimental Log】2015/10/20-22

8 Sample:CP0175 (1, B. ravenii), CP0176 (2, B. pengii), CP0177 (3, B. ravenii), CP0178 (4, B. ravenii), CP0179 (5, B. ravenii), CP0180 (6, B. ravenii), CP0181 (7, B. ravenii), CP0182 (8, B. pengii)。

DNA extraction:CTab (未秤重,目視約一片正常大小葉子,加入液態氮磨碎。) 總體積用50  ug去溶 (CP0177, CP0179, CP0180, CP0181: -3 ug, 其餘-2)(一次nanodrop, 一次Qbit)。未過Colum。

NanoDrop (曲老師實驗室)CP0175 (1, B. ravenii):237.4 ug/mL, 1.94 260/280, 1.54 260/230, CP0176 (2, B. pengii):223.2 ug/mL, 1.37 260/280, 0.6 260/230CP0177 (3, B. ravenii):70.8 ug/mL, 1.53 260/280, 0.59 260/230CP0178 (4, B. ravenii):117.3 ug/mL, 1.75 260/280, 0.77 260/230CP0179 (5, B. ravenii):39.8 ug/mL, 1.58 260/280, 0.48 260/230, CP0180 (6, B. ravenii):111.5 ug/mL, 1.77 260/280, 0.6 260/230CP0181 (7, B. ravenii):- ug/mL,  – 260/280, – 260/230CP0182 (8,B. pengii):78.8 ug/mL, 1.74 260/280, 0.77 260/230
(只有兩個曲線OK的: CP175, CP178。其餘不OK。)

Q bitCP0175 (1, B. ravenii):58.9 ug/mL, CP0176 (2, B. pengii):59.8 ug/mL, CP0177 (3, B. ravenii):77.2 ug/mLCP0178 (4, B. ravenii):52.5 ug/mLCP0179 (5, B. ravenii):59.3 ug/mLCP0180 (6, B. ravenii):50.9 ug/mLCP0181 (7, B. ravenii):66.7 ug/mLCP0182 (8,B. pengii):50.7 ug/mL
(都夠,都OK。)

Note: nanodrop好難懂喔。

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8 Sample:CP0170 (B. breviesepala), CP0172 (B. kawakamii), CP0173 (B. hayatana), CP0174 (B. hayatana), CP0183 (B. pengii), CP0183- (B. pengii), T1 (B. pengii), T2 (B. pengii)。(T1和T2,都已經冰過-80度,再退冰後發現便超黑,很糟糕。)

NanoDrop (曲老師實驗室)CP0170 (B. breviesepala):915 ug/mL, 1.86 260/280, 1.24 260/230CP0173 (B. hayatana):915 ug/mL, 1.91 260/280, 1.58 260/230。(其餘的都不OK。)

Q bitCP0170 (B. breviesepala):45.5 ug/mLCP0173 (B. hayatana):50.6 ug/mL

Note: 北大武山下山後,simple帶回來直接冰-80度。直接用液態氮。然後,南台灣小檗取樣太少了,有點悶。

【Experimental Log】2015/10/14-15

4 Sample:T1 (B. tarokoensis), T2 (B. tarokoensis), T4 (B. nantoensis), T5 (B. nantoensis)。

DNA extraction:CTab (未秤重,目視約一片正常大小葉子,加入液態氮磨碎。) 總體積用50 (-2) ug去溶。皆有過Colum。

NanoDrop (曲老師實驗室)沒結果。

Note:本來是要測試在保溫瓶裡冰8小時候,是否還能抽出DNA。使用人工氣候室裡面的小檗活體,B. tarokoensisB. nantoensis。但在CTAB結束前的結果都算不錯,在風乾時都能看到底部有一小半透明圓點(DNA),但過完colum,再去測濃度卻沒有東西。這次使用的colum是新的,另外買的,似乎在那一步有一點問題 (問群智學姊那問題出在哪裡)。想使用新的kit抽抽看sample。

Next:新kit (專抽給NGS用的品質)。測試:活體和標本樣品。(放八小時的實驗想等到北大武山回來後再試試,在去研海林道之前吧) sample:太魯閣小檗的不可用那些x2、B. mori,英國老爺爺的新種x1、B. apicola x1、活體 x2。

btw, 水,生日快樂。