【Experimental Log】2016/02/19-20

22 Samples:CP172 (B. kawakamii), CP174 (B. hayatana), CP184 (B. nantoensis), CP169 (B. alpicola), CP194 (B. tarokoensis), CP196 (B. schaaliae), CP197 (B. schaaliae), CP200 (B. aristatoserrulata), CP201 (B. aristatoserrulata), CP209 (B. kawakamii), CP210 (B. kawakamii), CP224 (B. kawakamii), CP225 (B. alpicola), CP226 (B. alpicola), CP227 (B. alpicola), CP233 (B. pengii), CP234 (B. pengii), CP235 (B. pengii), CP236 (B. pengii), CP237 (B. kawakamii)

DNA extraction:CTab (未秤重,目視約一片正常大小葉子,加入液態氮磨碎。) 總體積用50 ug去溶。[新來的!CP233, CP234, CP235, CP236, CP237,因為量多,就都各抽兩支 (-1和-2)。]

純化kit:Monarch™ PCR & DNA Cleanup Kit (5 μg) (NEB)

DNA定量:Qbit

20160219 Qbit結果:CP172 (B. kawakamii):39.8 ng/uL,, CP174 (B. hayatana):7.46 ng/uL, CP184-1 (B. nantoensis):20(20.2) ng/uL, CP184-2 (B. nantoensis):25 ng/uL, CP169 (B. alpicola):46.6 ng/uL, CP194 (B. tarokoensis):45 ng/uL, CP196 (B. schaaliae):42.2 ng/uL, CP197 (B. schaaliae):52.2 ng/uL, CP200 (B. aristatoserrulata):36 ng/uL, CP201 (B. aristatoserrulata):36.2 ng/uL, CP209 (B. kawakamii):51.2 ng/uL, CP210 (B. kawakamii):22 ng/uL, CP224 (B. kawakamii):91.8(92) ng/uL, CP225 (B. alpicola):35.6 ng/uL, CP226 (B. alpicola):0.142 ng/uL, CP227 (B. alpicola):29 ng/uL

20160220 Qbit結果:CP233-1 (B. pengii):too hight ng/uL, CP233-2 (B. pengii):110(112) ng/uL, CP234-1 (B. pengii):too hight ng/uL, CP234-2 (B. pengii):too hight ng/uL, CP235-1 (B. pengii):too hight ng/uL, CP235-2 (B. pengii):too hight ng/uL, CP236-1 (B. pengii):too hight ng/uL, CP236-2 (B. pengii):too hight ng/uL, CP237-1 (B. kawakamii):too hight ng/uL, CP237-2 (B. kawakamii):too hight ng/uL

CP172 (B. kawakamii):29 ng/uL, CP184 (B. nantoensis):45.2 ng/uL, CP224 (B. kawakamii):too hight ng/uL [這支CP172是突然找到,不知從何來的,突然出現的髒原液,沒有日期,和20160219那支CP172(原抽取時間為20151027) 的那管不同;CP148是CP148-1和CP148-2的濃縮成一管;CP224 20160219已測過,20160220重測]

結論:大家都很乖巧,表現也都不錯。只是20160219測得似乎大家都偏低 (怪)。又讓我很意外的是B. alpicola意外的難抽 (抽樣重測幾支,還算準確)。目前冰箱待過cleanup kit的sample都已經結束。下禮拜把活體抽抽完。

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