【Experimental Log】2016/02/22-23

16 Samples:CPL001 (B. kawakamii), CPL002 (B. nantoensis), CPL003 (B. nantoensis), CPL004 (B. mingetsensis), CPL005 (B. mingetsensis), CPL006 (B. breviesepala), CPL007 (B. tarokoensis), CPL008 (B. tarokoensis), CPL009 (B. tarokoensis), CPL013 (B. pengii), CPL014 (B. pengii), CPL015 (B. aristatoserrulata), CPL016 (B. aristatoserrulata), CPL017 (B. aristatoserrulata), CPL018 (B. breviesepala), CP227 (B. alpicola)

DNA extraction:CTab (未秤重,目視約一片正常大小葉子,加入液態氮磨碎。) 總體積用50 ug去溶。

純化kit:Monarch™ PCR & DNA Cleanup Kit (5 μg) (NEB)

DNA定量:Qbit

Qbit結果:CPL001 (B. kawakamii):too high ng/uL, CPL002 (B. nantoensis):70.2 ng/uL, CPL003 (B. nantoensis):80.0 ng/uL, CPL004 (B. mingetsensis):112 ng/uL, CPL005 (B. mingetsensis):106 ng/uL, CPL006 (B. breviesepala):too high ng/uL, CPL007 (B. tarokoensis):104 ng/uL, CPL008 (B. tarokoensis):99.4 ng/uL, CPL009 (B. tarokoensis):120 ng/uL, CPL013 (B. pengii):112 ng/uL, CPL014 (B. pengii):76.8(76.4) ng/uL, CPL015 (B. aristatoserrulata):too high ng/uL, CPL016 (B. aristatoserrulata):100 ng/uL, CPL017 (B. aristatoserrulata):98.4 ng/uL, CPL018 (B. breviesepala):91.4 ng/uL, CP227 (B. alpicola):85.0 ng/uL

結論:這次抽活體們。CPL1, CPL5, CPL7 實驗過程中有不小心掉落地面 (似乎沒有很大影響)

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