【Experimental Log】2016/02/28-29

6 Samples:(老師和漢堯學長當時的菲律賓採集之非常可怕的那袋) 5 (B. baradana), 9 (B. baradana),10 (B. baradana),13 (B. baradana),14 (B. baradana), CP238 (B. pengii)(雪怪學姊寄來的新鮮枝條)

DNA extraction:CTab (未秤重,目視約一片正常大小葉子,加入液態氮磨碎。) 總體積用50 ug去溶。

純化kit:Monarch™ PCR & DNA Cleanup Kit (5 μg) (NEB)

DNA定量:Qbit

Qbit結果: 5 (B. baradana):2.7 ng/uL, 9-1 (B. baradana):5.44 ng/uL,9-2 (B. baradana):4.84 ng/uL,10 (B. baradana):2.92(2.94) ng/uL,13 (B. baradana):0.49 ng/uL,14 (B. baradana):2 ng/uL, CP238-1 (B. pengii):too high ng/uL, CP238-2 (B. pengii):102 ng/uL


 

14 Samples:(漢堯學長當時抽的菲律賓那些原液)S1 (B. baradana), S2 (B. baradana), S3 (B. baradana), S5 (B. baradana), S6 (B. baradana), S7 (B. baradana), S8 (B. baradana), S9 (B. baradana), S10 (B. baradana), S11 (B. baradana), S12 (B. baradana), S13 (B. baradana), S14 (B. baradana), S15 (B. baradana)

純化kit:Monarch™ PCR & DNA Cleanup Kit (5 μg) (NEB)

DNA定量:Qbit

Qbit結果:S1 (B. baradana):ˊ62.4 ng/uL, S2 (B. baradana):22.8 ng/uL, S3 (B. baradana):25.2 ng/uL, S5 (B. baradana):48.4 ng/uL, S6 (B. baradana):49.6 ng/uL, S7 (B. baradana):24.2 ng/uL, S8 (B. baradana):27.6 ng/uL, S9 (B. baradana):20 ng/uL, S10 (B. baradana):25.6 ng/uL, S11 (B. baradana):81.2(80.6) ng/uL, S12 (B. baradana):20.6 ng/uL, S13 (B. baradana):65.4 ng/uL, S14 (B. baradana):39.6 ng/uL, S15 (B. baradana):36.8 ng/uL (注意!我只有溶20 ug,由於原始濃度過低,只好降低體積求濃度。)

 

結論:這是B. baradana之日。其實有抽到足夠濃度的Sample (但大部分狀況都非常糟糕)。接下來會跑膠,看DNA長度破碎問題。也和熊爺爺再要這個種了。希望能加油。

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