【Experimental Log】2016/03/10

8 Samples:D1 (B. baradana), D5 (B. baradana), D6 (B. baradana), D7 (B. baradana), CP104 (B. breviesepala), CP170 (B. breviesepala), CP173 (B. hayatana), 745 (B. aristatoserrulata)

純化kit:Monarch™ PCR & DNA Cleanup Kit (5 μg) (NEB) [過漢堯學長抽的D1, D5, D6, D7,只溶20 μL]

DNA定量:Qbit [漢堯學長抽的D1, D5, D6, D7,最後溶20 μL]

Qbit結果:D1 (B. baradana):73.6 ng/uL, D5 (B. baradana):39.4(39.2) ng/uL, D6 (B. baradana):112 ng/uL, D7 (B. baradana):79.2 ng/uL

跑膠結果

20160310跑膠v1

結論:發現漢堯學長抽的B. baradana D6, D7,似乎還用!(意外的驚喜) 我的(CP系列)片段大小都還算大。學長的sample(745)smear嚴重。

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