【Experimental Log】2016/03/19

10 Samples:CPL6 (B. breviesepala), CP221 (B. hayatana), CP237-1 (B. kawakamii), CP117 (B. ravenii), CP181 (B. ravenii), CP132 (B. schaaliae), B318a (B. baradana), 武1 (B. wuyiensis), 武2 (B. wuyiensis), 武3 (B. wuyiensis)

純化kit:Monarch™ PCR & DNA Cleanup Kit (5 μg) (NEB) [CP117 (B. ravenii), CP181 (B. ravenii), 武1 (B. wuyiensis), 武2 (B. wuyiensis), 武3 (B. wuyiensis),原來CP117和CP181只是要跑膠,赫然發現這兩支沒有過純化,原液顏色汙濁,故立馬過clean up,在測Qbit。]

DNA定量:Qbit [CPL6 (B. breviesepala), CP237-1 (B. kawakamii), B318a (B. baradana), 武1 (B. wuyiensis), 武2 (B. wuyiensis), 武3 (B. wuyiensis), CP117 (B. ravenii), CP181 (B. ravenii)]

Qbit結果:CPL6 (B. breviesepala):57.2 ng/uL, CP237-1 (B. kawakamii):68.8(69.4) ng/uL, B318a (B. baradana):56.8 ng/uL, 武1 (B. wuyiensis):38.6 ng/uL, 武2 (B. wuyiensis):34.4 ng/uL, 武3 (B. wuyiensis):55 ng/uL, CP117 (B. ravenii):21.6 ng/uL, CP181 (B. ravenii):12.4 ng/uL

跑膠結果:[CPL6 (B. breviesepala), CP221 (B. hayatana), CP132 (B. schaaliae), 武1 (B. wuyiensis), 武2 (B. wuyiensis), 武3 (B. wuyiensis)]

20160319跑膠

結論:跑膠OK!但是但是但是但是,非常可怕的漏網之魚→CP117 (B. ravenii):21.6 ng/uL, CP181 (B. ravenii):12.4 ng/uL,原來在20151022測得77.2和66.7!濃度都是髒東西,兩支皆不能使用,過完Cleanup才發現,濃度不夠!緊急重抽!(幸好還有活體!)

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