【Experimental Log】2016/03/28

17 Samples:CP227 (B. alpicola), CPL15 (B. aristatoserrulata), CPL16 (B. aristatoserrulata), CP191 (B. breviesepala), CPL18 (B. breviesepala), CP221 (B. kawakamii), CP214 (B. kawakamii), CPL10 (B. ravenii), CPL11 (B. ravenii), CPL12 (B. ravenii), CPL3 (B. nantoensis), CP231-1 (B. pengii), CP232-1 (B. pengii), CPL13 (B. pengii), CPL8 (B. tarokoensis), CPL9 (B. tarokoensis), CPL4 (B. mingetsensis)

DNA extraction:CTab (未秤重,目視約一片正常大小葉子,加入液態氮磨碎。) 總體積用50 ug去溶。[CPL10 (B. ravenii), CPL11 (B. ravenii), CPL12 (B. ravenii)]

DNA定量:Qbit [CPL10 (B. ravenii), CPL11 (B. ravenii), CPL12 (B. ravenii), CPL15 (B. aristatoserrulata),由於在20160223抽的那支,濃度too high,所以這次稀釋一半後再測濃度。]

Qbit結果:CPL15 (B. aristatoserrulata):69.4 ng/uL, CPL10 (B. ravenii):50.2 ng/uL, CPL11 (B. ravenii):59.2 ng/uL, CPL12 (B. ravenii):too high

跑膠:CP227 (B. alpicola), CPL15 (B. aristatoserrulata), CPL16 (B. aristatoserrulata), CP191 (B. breviesepala), CPL18 (B. breviesepala), CP221 (B. kawakamii), CP214 (B. kawakamii), CPL10 (B. ravenii), CPL11 (B. ravenii), CPL12 (B. ravenii), CPL3 (B. nantoensis), CP231-1 (B. pengii), CP232-1 (B. pengii), CPL13 (B. pengii), CPL8 (B. tarokoensis), CPL9 (B. tarokoensis), CPL4 (B. mingetsensis)

20160328跑膠

結果:跑膠結果皆OK!

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